Smyrna (Revelation 2:8-11) - the church that would suffer persecution (2:10).
Pergamum (Revelation 2:12-17) - the church that needed to repent (2:16).
Thyatira (Revelation 2:18-29) - the church that had a false prophetess (2:20).

Unfortunately of the “original” Smyrna nothing is left. Yet you may wish to visit the Church of Saint Polycarp, the first Christian Martyre. Then departure for Pergamon :
In the subject of our tour, a visit to the Red Basilica, a redbrick edifice below the acropolis, originally a temple to Serapis converted into a basilica by the Byzantine.A visit to the Acropolis (theater and altar of Zeus) as well as to the Esculapeion (medical center and temple dedicated to the god of medicines) are surely recommended.On the way to Salihli, a detour for a short visit to Thyateira (not much to be seen) is possible.
Overnight Salihli

Sardis (Revelation 3:1-6) - the church that had fallen asleep (3:2).
Philadelphia (Revelation 3:7-13) - the church that had endured patiently (3:10).

Today we will pay a visit to Philadelphia (unfortunately very poor in christian remainings) and proceed to Sardis. Though Sardis does not owe any christian remainings except for a small brick Byzantine church, a visit to the synagogue, gymnasium and bath, the marble court is worth being paid. We then take the way to Pamukkale for overnight.
Overnight Pamukkale

Laodicea (Revelation 3:14-22) - the church with the lukewarm faith (3:16).
In the morning, we will concentrate on the newly excavated site of Laodikea : you will see amongst others a Baptist, sacred road, 2 theaters..
In the afternoon, we will return to Pamukkale to visit Hierapolis (Martyrium of Saint Philips, theater, necropolis…) and the travertines of Pamukkale.
At the end of the tour, we depart for Kusadasi (overnight)
Overnight Kusadasi

Ephesus (Revelation 2:1-7) - the church that had forsaken its first love (2:4).

Today we will discover the most popular site of the Seven Churches; Ephesus.We will start the day with a visit to the Shrine of Virgin Mary : the house whe-re the Virgin Mary lived is today the chapel open also to Muslim pilgrims for whom Mother Mary is also a saint. Ephesus' religious importance was firstly due to the worship to Artemis who was later on replaced by the worship to Virgin Mary; then to the preaches of Saint Paul to the Jewish community. As one of the Seven Churches of Revelation, Ephesus was also a venue for an ecumenical council : in 431 (anathemization of the Nestorian heresy) in the Church of Saint Mary (on the right of the main entrance), constructed between the IInd and IVth A.D., originally a Roman warehouse. The visit of Ephesus can be prolounged with a classical tour of the site. The church of Saint John (entrance through the Gate of Persecution) is traditionally know as the burial place of Saint John the Evangelist who died here around 100 A.D. Colonnades and walls have been re-erected just to give an idea of the magnificence of the building at its origins. At the end of the tour, return to Kusadasi for overnight.
Overnight Kusadasi

Depending the ferry schedules…
In the morning, we take the ferry to Samos (port of Vathi) whe-re we will spend the day before taking the afternoon ferry to Patmos (from the port of Pythagorion).
Overnight in Patmos

Visit Patmos : Jerusalem of the Aegean is one way of describing PatmosPatnos, as it was referred to in a 5th century inscription. It was here that St. John the Theologian was exiled between 95 and 97 A.D. and was inspired to write the Book of Revelation. The islands shape and its 63km coastline offers a wide ion of beaches, from sandy and organized shores with umbrellas and beach bars to solitary coves. The choice of whe-re to enjoy some of the clearest, refreshing and tranquil waters of the Aegean Sea is yours. If you're arriving with a private boatsimply renting one, you're in for an unforgettable experience and not only on our island, for Patmos has many small and larger neighboring islands making short distance excursions enticing . Recommended tours on Patmos (check our Excursion pages for details) 
Half Day Monastery Tour
Half Day Patmos Highlights
In the evening, return to Samos for overnight

As per ferry schedules (or your wish to spend some time on Samos), take the morningafternoon ferry back to Kusadasi… from whe-re you will be transferred to Izmir airport and then fly back to Istanbul.